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Figure Caption
Fig. 5 Localization of β-cat at the AJs requires Chp signaling.
(A) A middle sagittal plane of the embryo at 60% epiboly immunostained with β-cat is also derived from the Z stack. β-cat was no longer maintained at the AJs in the EVL and was found predominately in cytoplasm. Scale bars represent 20 μm. (B) Immunostaining of β-cat comparing the EVL and DEL of un–injected control and Chp morphant. Levels of anti-β-cat staining at AJs were severely reduced in Chp morphants. The delocalized β-cat protein was detected within intracellular structures, but no nuclear enrichment was observed relative to controls.
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