ZFIN Image: Ko et al., 2010, Fig. 4

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Figure Caption

Fig. 4 Positional Cloning of bromi
(A) bromi was mapped to a 2.3 Mb region on mouse chromosome 10 by analysis of recombinants (recombination events, out of 219 opportunities scored for each marker, are listed). This interval contained six known and predicted genes, which were fully sequenced in bromi and parental (C57BL/6J) strains. (B and C) An A-to-G splice acceptor mutation at the intron 10/exon 11 junction of C6orf170 was identified in bromi samples (trace shown in [C]), and sequencing of RT-PCR products showed that bromi mutants use a cryptic splice acceptor site 13 base pairs downstream.
(D) Western blotting of WT and bromi mutant embryonic extracts with polyclonal antisera against the Bromi protein.

Acknowledgments

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Reprinted from Developmental Cell, 18(2), Ko, H.W., Norman, R.X., Tran, J., Fuller, K.P., Fukuda, M., and Eggenschwiler, J.T., Broad-Minded Links Cell Cycle-Related Kinase to Cilia Assembly and Hedgehog Signal Transduction, 237-247, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell