ZFIN Image: Brown et al., 2010, Fig. 2

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Figure Caption

Fig. 2 Electrophoretic mobility shift assays on Rx3 predicted binding sites. Upper panels: in the sequence alignment between the mouse RAX binding site and predicted Rx binding sites the upstream (A) and intronic (B) regions in 4 fish species, conserved nucleotide positions are highlighted in blue. Mutations made within the homeobox core binding site are highlighted in red. Lower panels: Rx3 predicted binding sites in zebrafish nlcam were tested by EMSA. Rx3 specifically binds to the oligonucleotides containing the RAX motif, but not to mutated versions. This binding can be competed away by increasing levels of unlabelled oligonucleotide.

Acknowledgments

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Reprinted from Developmental Biology, 339(1), Brown, K.E., Keller, P.J., Ramialison, M., Rembold, M., Stelzer, E.H., Loosli, F., and Wittbrodt, J., Nlcam modulates midline convergence during anterior neural plate morphogenesis, 14-25, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.