Swimbladder inflation is affected in the absence of blood circulation. (A - C) EGFP expression in swimbladder epithelium in control, clo-/- mutant, and Tnnt2 morphants on the background of Et(krt4:EGFP)sq33-2 at 5 dpf. (D - F) EGFP expression in swimbladder outer mesothelium in control, clo-/- mutant, and Tnnt2 morphant on the background of Et(krt4:EGFP)sqet3 at 5 dpf. All mutants and morphants failed to inflate their swimbladder by 5 dpf. Abbreviation: sb, swimbladder. Scale bars: 250 μm.
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