ZFIN Image: O'Brien et al., 2009, Fig. 2

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Figure Caption

Fig. 2 The Capacity for Reinnervation Diminishes during Development

Axotomy at 30 hpf (A–C), 54 hpf (D–F), or 78 hpf (G–I). Left two columns are confocal projections. Scale bars represent 50 μm. Yellow arrowheads indicate site of axotomy, and olive overlay marks the territory that was denervated after axotomy. Blue dashed boxes indicate the area represented in rightmost panels (C, F, and I), which show 3D reconstructions of the same axon before axotomy (green) and 12 hr after axotomy (red), aligned at the shared branchpoint most proximal to the axotomy site.

(J) Quantification of the average area reinnervated by injured axons, calculated as surface area of regenerated axon in denervated territory divided by surface area of denervated territory.

(K) Quantification of average new axon growth that entered the denervated territory, calculated as length of new growth in denervated region divided by total length of new growth. Error bars represent ± standard error of the mean (SEM). See Table S1 and Movies S2–S4.

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Acknowledgments

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