ZFIN Image: Rieger et al., 2009, Fig. 6

Image

Figure Caption

Fig. 6 Migratory pac^-/-R cerebellar GCs show reduced contact stability and adherens junctions.

(A–D) Comparison of contact duration between individual gata1:GFP GCs in time-lapse sequences (white arrowheads) reveals that lack of Cadherin-2 in pac^-/-R GCs significantly reduces the duration of stable cell-cell contacts (E, WT: 95.3±10.9 min versus pac^-/-R: 36.8±5.6 min, n = 29). (F–H) TEM analysis reveals that AJs between pac^-/-R cerebellar neurons are largely absent (G), while numerous AJs are found between individual WT cerebellar neurons (F). (H) pac^-/-R cerebellar neurons appear disorganized (compare with Figure 1J). (I) Quantification of TEM images (3.8±0.9 AJs, n = 10, in WT versus 0.3±0.2 AJs, n = 7, in pac^-/-R-cerebella).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.