IMAGE

Fig. S6

ID
ZDB-IMAGE-091113-37
Source
Figures for Joshi et al., 2009
Image
Figure Caption

Fig. S6 APP695 and APP-CΔ18 are present at the plasma membrane in HEK 293 cells. Confocal images of HEK 293 cells transiently transfected with pCS2 + APP695 or the deletion mutant pCS2 + APP-CΔ18. The cells were fixed in paraformaldehyde and probed with 6E10 antibody against the Aβ region or with 22C11 antibody against the extracellular region of APP, followed by an anti-mouse secondary conjugated with Alexa 594. The immunofluorescence images show that full-length APP695 (A) or CΔ18 truncation protein (B) can be detected at the plasma membrane when detected with 6E10 antibody. The immunofluorescence pattern is indistinguishable between APP695 and CD18 (white arrowheads). 22C11 antibody that recognizes the N-terminal domain of APP gave a similar staining pattern (C, D).

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Reprinted from Developmental Biology, 335(1), Joshi, P., Liang, J.O., Dimonte, K., Sullivan, J., and Pimplikar, S.W., Amyloid precursor protein is required for convergent-extension movements during Zebrafish development, 1-11, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.