|ZFIN ID: ZDB-IMAGE-091016-44|
Fig. S1 A) Alignment of Dnct1 from zebrafish (top sequence) versus human (bottom sequence) for the region of the dominant negative protein. Alignments were generated in MegAlign (DNAstar, V6) using the Lipman-Pearson protein algorithm with 0 gap penalty. Overall sequence identity between the proteins is 65%. within specific domains, however, identity is higher: Microtubule binding domain/Cap-Gly region, 86% identity (green); dynein motor binding domain, 84% identity (yellow); dynein intermediate chain binding domain, 80% identity (blue).
B) The dominant negative p150-GFP labels spindle structures at mitosis.
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