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Fig. 3 GAB and 5′HS4 insulators decrease the position effect. A: F0 embryos injected with a Tol2 transposon containing the gata2 minimal promoter driving green fluorescent protein (GFP). The GFP background expression is due to position effect in independent random insertions and was observed in a large number of cells in 40% of the injected embryos (n > 100). B: Most of this background is lost in embryos injected with similar construct flanked GAB and 5′HS4 insulators, as we observed GFP expression in only 16% of the injected embryos (n > 100) and in a much reduced number of cells.
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