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Fig. 2 Partial VE-cadherin knockdown causes vascular instability and hemorrhages.
(A–D) Bright field images of 52 hpf embryos. Lateral views, anterior to the left. Embryos injected with low doses (0.8 ng) of VE-cadherin morpholino (Low VEcadMO) develop single (C) or multiple hemorrhages (B and D) that were evident at 52 hpf and appeared in the brain (arrows) and/or aortic arches (arrowheads). No or very rare hemorrhages were observed in embryos injected with mismatch control morpholino (ctrlMO) at the same concentration (A). (E, F) Confocal images of Tg(flk1:EGFP) embryos at 54 hpf. Dorsal views, anterior to the left. In embryos injected with ctrlMO (E), central arteries (CtA) extend from the basilar artery (BA) towards forebrain and midbrain, and drain into the primordial hindbrain channel (PHBC). In contrast, in Low VEcadMO injected embryos (F) only few CtAs are formed, and those which are present fail to fuse to BA and lack lumen (asterisks).