Fig. 2

Fig. 2 Cell-autonomous detection of the β-lactamase marker. (A) One-cell-stage embryos were injected with CCF2 and later at the 16-cell stage one of the marginal blastomeres was injected with β-lactamase mRNA. At this stage the cytoplasm of the marginal blastomeres is not separated allowing the mRNA to diffuse and to be translated in more than 1/16 of the cells. (B, C) One-cell-stage embryos were injected with CCF2 and later at the 64-cell stage one of the blastomeres was coinjected with β-lactamase mRNA and with rhodamine dextran as a lineage marker. At 30% epiboly the yolk was removed and a flat preparation of the embryo was visualized at a high magnification using the CCF2 filter set (B) or with a filter set for rhodamine detection (C).