Representative transmitted light images of 48 hours-old zebrafish larvae illustrating the phenotype under different experimental conditions. (A) Wild type (WT), non injected larvae. (B) Knockdown larva, injected with AMO targeting the translation start site of Ap1s1 (N-terminal). (C) Larva rescued by co-injection of AMO and human WT mRNA (HmRNA). (D) Larva injected with a mismatch AMO. (E) Larva rescued by co-injection of AMO and -9bp Human mRNA. (F) larva co-injected with AMO and -exon3 HmRNA. (G) Knockdown larva, injected with AMO targeting the acceptor splice site (intron 2) of Ap1s1.
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