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Fig. 6

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Fig. 6 Expression of Mutants Matriptase-2 in Zebrafish Affects Hemoglobinization of Erythrocytes

(A) Zebrafish embryos were injected with control (zcontrol MO) and matriptase-2 morpholino (zmt2 MO). Fourty-eight hours post injection, embryos were stained with o-dianisidine to detect hemoglobinized cells.

(B) Embryos as in (A) were homogenized, and RNA was extracted as described in Experimental Procedures. RT-PCR was performed using oligonucleotides specific for matriptase-2 (zmt2) and for the elongation factor 1α (zef1α). Four-hundred fifty embryos were injected, and the survival rate was 85%.

(C) Zebrafish embryos were injected with matriptase-2wt (MT2wt), MT2R774C, and MT2MASK expressing vectors, in the presence (+Fe) or not (-Fe) of iron dextran. At 48 hr after fertilization embryos were stained with o-dianisidine to detect hemoglobinized cells. Representative pictures of the injected fishes (200 for each construct, made in triplicate) are shown.

(D) MT2 levels were assayed by western blot analysis.

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Reprinted from Cell Metabolism, 8(6), Silvestri, L., Pagani, A., Nai, A., De Domenico, I., Kaplan, J., and Camaschella, C., The Serine Protease Matriptase-2 (TMPRSS6) Inhibits Hepcidin Activation by Cleaving Membrane Hemojuvelin, 502-511, Copyright (2008) with permission from Elsevier. Full text @ Cell Metab.