Fig. 5

Fig. 5 Functional redundancy of wnt1 and wnt10b. (A) Confocal image of GFP expression from PAC 176DG16. Lateral view of head of 24-hpf embryo to illustrate strong expression in the tectum (arrow). Expressing cells are also found in the MHB and hindbrain (arrowheads). (B–I) Probe used is indicated in upper right of each panel; genotype is in lower right. (B–D) Rescue of pax2.1 expression in Df^w5 embryos by PAC injection. (B) Wild type embryo. (C) Df^w5 homozygote to show loss of pax2.1 in ventral MHB (arrow). (D) Df^w5 homozygote with restored ventral pax2.1 expression (arrow) due to injection of PAC176ΔG16. (E–I) Phenotypes induced by wnt1 and wnt10b morpholinos. (E) Control uninjected embryo. pax2.1 expression observed in embryos injected with either wnt1-MO or wnt10b-MO is indistinguishable from this example. Note that MHB pax2.1 expression appears normal (arrow). (F) Embryo coinjected with both wnt1-MO and wnt10b-MO. Note loss of ventral pax2.1 expression as seen in Df^w5 homozygotes (compare with embryo in C). (G) en2 expression in control uninjected 24-hpf embryo. Embryos injected with wnt10b-MO are indistinguishable from this example. (H) Weak reduction in en2 expression seen in ∼30% of embryos injected with wnt1-MO. Note reduction localized to the ventral MHB (arrow). (I) Stronger reduction in en2 expression upon wnt1-MO or wnt1 + wnt10b- MO injection. Compare this phenotype with the Df^w5 embryo in Fig. 4H.