ZFIN Image: Stenkamp et al., 2000, Fig. 5

Image

Figure Caption

Fig. 5 Recruitment of rods and red cones in wild-type and syu^t4 embryos and in shh/twhh antisense-injected wild-type embryos. Eyes were from 75-hpf embryos, hybridized with GFrod (red color; arrows) and GFred (dark color; arrowheads). (A) Wild-type embryo. Image is a superimposed projection of several focal planes (using the “apply image” function in Adobe PhotoShop), in bright-field and epifluorescence optics. Scale bar (applies to A and B), 50 μm; v, ventral. (B) Eye of embryo injected with shh/twhh antisense oligos. (C) syu^t4 embryo. B and C are superimposed epifluorescent and bright-field images, but from a single focal plane that contained all labeled photoreceptors. Scale bar in C, 50 μm.

Acknowledgments

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Reprinted from Developmental Biology, 220(2), Stenkamp, D.L., Frey, R.A., Prabhudesai, S.N., and Raymond, P.A., Function for hedgehog genes in zebrafish retinal development, 238-252, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.