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Fig. S6

ID
ZDB-IMAGE-080829-27
Source
Figures for Liu et al., 2008
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Figure Caption

Fig. S6

RFP expression analysis at 20 hpf for k-ras-5′UTR-RFP injected embryos, indicating the targeting specificity of k-ras morpholino antisense oligo.

(A) Embryo injected with k-ras-5′UTR-RFP/PCS (red fluorescent protein reporter was down stream of K-ras 5′UTR and was cloned into PCS2 vector) construct, showing strong RFP signal.

(B) Embryo co-injected with k-ras-5′UTR-RFP/PCS and k-ras-MO1, showing very weak RFP signal, indicating the blockage of RFP protein expression by k-ras-MO1.

(C) Embryos from different treatments, showing the different RFP strength under the same exposure. These embryos were (i), injected with k-ras-5′UTR-RFP/PCS alone; (ii), co-injected with k-ras-5′UTR-RFP/PCS and k-ras-MO1; and (iii), wild type embryo with no injection.

Acknowledgments
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