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Fig. 8 nrg1 and nrg2a act redundantly during DRG neuron formation. (A,B) Elav1 antibody labeling at 4 dpf. Co-injection of nrg1 and nrg2a MOs resulted in absence of DRG neurons (B), whereas DRG neurons are normal in controls (A). (C-H) Whole-mount wild-type (C,E,G) and nrg1 plus nrg2a MO-injected embryos (D,F,H) labeled with crestin riboprobe at 24 hpf (C,D), 27 hpf (E,F) and 30 hpf (G,H). Like erbb3b and erbb2 mutants, double MO-injected embryos have normal early NC migration (C,D) but, at later stages, NC migration is disrupted (E-H). Scale bar: 20 μm.
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