URRs Are Required for Dnd1 to Efficiently Repress miR-430-Mediated Nanos Inhibition (A) RNA containing the DsRed open-reading frame fused to the wild-type nanos1 3′UTR (3′nos1wt) was coinjected into one-cell-stage zebrafish embryos together with RNA containing the gfp open-reading frame fused to different versions of the nanos1 3′UTR. The different nanos1 3′UTRs that were used are shown above; mutations are marked in red. The ratio between the signal intensity provided by GFP whose open-reading frame was fused to either one of the nanos UTRs was divided by that originating from DsRed that was fused to the wild-type nanos RNA UTR. Representative single cells are shown in the right panels. (B) An experimental setting similar to that described in (A) was used to examine the function of nanos UTR containing a combination of mutations in the miR-430 and putative Dnd-binding sites. The different nanos1 3′UTRs that were used are shown above; mutations are marked in red. The ratio between the signal intensity provided dividing the signal intensity of GFP by that of DsRed whose open-reading frame was fused to the wild-type nanos RNA UTR. Representative single cells are shown in the right panels. Error bars depict the standard error of the mean (SEM); p value was calculated using Ttest.
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