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Fig. 7 Conserved M1 and M2 motifs are both required to drive gfp expression in the notochord. (A) Embryo injected with the gfp-reporter control, containing 80 bp of the ctgf basal promoter (TATA box). This promoter is inactive without addition of an upstream CRM. (B, C, D) gfp-reporter constructs under the control of M1 and M2 motifs from ctgf up1 (B), sox9a E1 (C) or id1 A8 (D) drive gfp expression in notochord cells. (E, F) Three copies of either the ctgf M1 motif (E) or of the ctgf M2 motif (F) are not sufficient for gfp-reporter expression, indicating that each of the elements alone is not sufficient and that the combination of the two elements specifies notochord expression. Embryos are 26 hpf old and are oriented anterior to the left and dorsal up. Numbers in the schematic drawing to the left indicate positions of fragments relative to the ATG of the respective linked gene. Scale bar = 250 μm.
Reprinted from Developmental Biology, 318(2), Rastegar, S., Hess, I., Dickmeis, T., Nicod, J.C., Ertzer, R., Hadzhiev, Y., Thies, W.G., Scherer, G., and Strähle, U., The words of the regulatory code are arranged in a variable manner in highly conserved enhancers, 366-377, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.