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Fig. 1 Oscillation of clock components under a LD cycle. RNase protection analysis of Per1, Per2, Per3, Clock, Bmal1, and Bmal2 gene expression in Z3 cells entrained for 5 days under a 12:12 LD cycle. RNA was harvested at the indicated ZTs during day 6 of the LD cycle. The bar above indicates light (white) and dark (black) periods. tRNA serves as a negative control reaction (t). Relative amounts of total RNA used for each sample are displayed (RNA). The ZT0 and ZT24 represent two independent samples that were harvested at the represented dark to light transition points.
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