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Fig. 5 Entrainment of Z3 cells to an alternate LD cycle. RNase protection analysis of Per1, Per2, Per3, Clock, Bmal1, and Bmal2 gene expression in Z3 cells entrained for 5 days under a 12:12 LD cycle. After day 5 of entrainment, the LD cycle was inverted causing a 12-h extension of the dark phase. RNA was harvested at the indicated ZTs. The bar above indicates light (white) and dark (black) periods. tRNA serves as a negative control reaction (t). Relative amounts of total RNA used for each sample are displayed (RNA).
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