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Fig. 4 Duration of circadian oscillation in constant darkness. RNase protection analysis of Per1, Per2, Per3, Clock, Bmal1, and Bmal2 gene expression in Z3 cells entrained for 5 days under a 12:12 LD cycle and the placed in constant darkness. RNA was harvested at the indicated CTs for a period of 4 days. The bar above indicates the dark (black) period. tRNA serves as our negative control reaction (t). Relative amounts of total RNA used for each sample are displayed (RNA).
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