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Fig. S6 Parapineal ablation causes a substantial reduction in epithalamic asymmetries. (a-f) Dorsal views of the epithalamus in larvae in which parapineal ablation was performed at 24–28 hpf, and gene expression and neuropil organization were assessed at 4 dpf. (a, b) In the parapineal-ablated larva, lov expression is substantially reduced in the L habenula to levels similar to the R habenula. However, a small, asymmetric, medial expression domain is retained (arrow in (b)). (c, d) ron expression appears bilaterally symmetric in the parapineal-ablated larva. (e, f) Anti-acetylated tubulin immunostaining reveals a considerable reduction in the size of the asymmetric dorsomedial neuropil domain in the L habenula after parapineal ablation. However, a small medial 'stump' is retained (arrows). All panels show dorsal views, anterior top.