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Fig. 8 Human Tbx20 T-box domain can functionally substitute for mab-9. (A) WT hermaphrodite carrying the evIs82b unc-129::GFP reporter, expressed in DA and DB motor neurons plus other neurons in the head and tail. (B) mab-9(e2410) animal carrying the unc-129::GFP reporter. The expression of unc-129::GFP is specifically reduced in motor neurons and is only observed very weakly in a couple of neurons at the anterior and posterior. (C) mab-9(e2410) animal carrying the genomic mab-9 construct pAW118 as well as the unc-129::GFP reporter. Complete rescue of the unc-129::GFP neuronal expression defect is observed, with the animal looking very similar to WT. (D) mab-9(e2410) animal carrying the mab-9 minigene construct pAW230 (ouEx63) in addition to the unc-129::GFP reporter. Again, complete rescue of the unc-129::GFP neuronal expression defect is observed. (E) mab-9(e2410) animal carrying the mab-9/HsTBX20 chimeric minigene construct pAW271 (ouEx62) as well as the unc-129::GFP reporter. Complete rescue of the neuronal phenotype is again observed. Typical phenotypes are shown. Posterior is to the right on all panels.
Reprinted from Developmental Biology, 317(2), Pocock, R., Mione, M., Hussain, S., Maxwell, S., Pontecorvi, M., Aslam, S., Gerrelli, D., Sowden, J.C., and Woollard, A., Neuronal function of Tbx20 conserved from nematodes to vertebrates, 671-685, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.