ZFIN Image: Köster et al., 2001, Fig. 2

Image

Figure Caption

Fig. 2 Gal4-VP16-mediated expression in comparison to conventional DNA-construct expression. Lateral views of the hindbrain (A, C, E) and trunk (B, D, F) of CMV-lyn-injected (A, B), CMV-GVP/Ulyn-coinjected, and EF-GVP/Ulyn-coinjected embryos at 3 dpf. Pictures represent pseudocolored composites of single optical sections using confocal microscopy. EGFP-expressing cells are displayed in green. Embryos where counterstained with Bodipyceramide TexasRed (Molecular Probes) displayed in red to visualize overall morphology. Note the precise membranelocalization of the lynEGFP-fluorescence in expressing cells (e.g., neuron in A or muscle cells in D). Abbreviations: nc, notochord; nt, neural tube; otv, otic vesicle; rh, rhombencephalon.

Acknowledgments

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Reprinted from Developmental Biology, 233(2), Köster, R.W. and Fraser, S.E., Tracing transgene expression in living zebrafish embryos, 329-346, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.