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Fig. 3 Comparison of calcein staining with Alcian blue staining. (A, C, E) Calcein labeling of calcified structures in the head, trunk, and tail regions of a 23-dpf embryo. (B, D, F) Alcian blue staining in the head, trunk, and tail regions of a 23-dpf embryo.
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Reprinted from Developmental Biology, 238(2), Du, S., Frenkel, V., Kindschi, G., and Zohar, Y., Visualizing normal and defective bone development in zebrafish embryos using the fluorescent chromophore calcein, 239-246, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.