Visualization of calcified skeletal structures in developing zebrafish embryos at 5, 6, 7, 9, 11, 14, 16, and 21 dpf by calcein staining. (A, B) Ventral view of the head skeleton of day-7 (A) and -11 (B) embryos labeled with calcein. m, Meckel’s cartilage; pq, palatoquadrate; e, ethmoid plate. (C, D) Side view of the head skeleton of day-7 (A) and -11 (B) embryos labeled with calcein. n, notochord; c, cleitrum.
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