IMAGE

Fig. 1

ID
ZDB-IMAGE-080422-5
Genes
Source
Figures for Chung et al., 2008
Image
Figure Caption

Fig. 1 Initial Position of Tg(ins:GFP)-Expressing Cells

12- (A–C, and F), 15- (D–E, and I), and 18- (G–H) somite stage (corresponding to 15, 16.5, and 18 hpf, respectively) Tg(ins:GFP) embryos showing GFP (green; [A–B and D–I]), TOPRO (white; [B, E, and H]), β-catenin (red; [B, E, and H]) and Tg(sox17:DsRed)s903 (red; [F and I]) staining. Dorsal views of fixed embryos (A, D, and G), and confocal images of transverse sections (B, E, and H) and of ventral views (F and I). (A) One Tg(ins:GFP)-expressing cell (arrow) is present in the endoderm of this 12-somite stage embryo. Cells in the anterior neural tube (arrowhead) also express the ins:GFP transgene. (B) The Tg(ins:GFP)-expressing cell (arrow) is located in the medio-lateral axis between the notochord and left somite. (C) Schematic diagram of a transverse section showing Tg(ins:GFP)-expressing cells (green) within the endodermal sheet (yellow), as well as the notochord (red), somites (blue), neural tube (gray), and LPM (purple) at the 12-somite stage. Note that the endoderm consists of a monolayer of cells located at the ventral-most side of the embryo. (D, E, G, and H) At the 15- (D and E) and 18- (G and H) somite stage, Tg(ins:GFP)-expressing cells (arrows) lie bilateral to the notochord. Cells in the anterior neural tube (arrowhead) also express the ins:GFP transgene. (E and H) Mesenchymal cells (arrowheads) have migrated toward the midline. (F and I) At the 12- (F) and 15- (I) somite stage, gaps (asterisks) can be observed in the midline of the fusing endodermal sheet (red). Most Tg(ins:GFP)-expressing cells (green; arrows) are located bilateral to the notochord.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 14(4), Chung, W.S., and Stainier, D.Y., Intra-endodermal interactions are required for pancreatic beta cell induction, 582-593, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell