IMAGE

Fig. S4

ID
ZDB-IMAGE-080422-15
Source
Figures for Chung et al., 2008
Image
Figure Caption

Fig. S4 Smoothened Function in Adaxial and Lateral Floor Plate Cells Does Not Appear to Be Required for the Induction of Dorsal Pancreatic β-Cells
(A) Schematic diagram of the cell transplantation protocol. Wildtype Tg(ins:GFP) donor cells were transplanted into wildtype or smo mutant Tg(ins:GFP) hosts. (B-G) Transverse confocal images of wildtype (B and E) and smo mutant (C, D, F and G) hosts stained for GFP (green) and β-catenin (white) at 18 hpf. Wildtype donor cells are labeled with rhodamine dextran (red). (B and E) Regardless of the position of the wildtype donor cells, Tg(ins:GFP) expression is unaffected in wildtype hosts. (C, D, F and G) 13 Tg(ins:GFP) expression is not rescued in smo mutant hosts when wildtype donor cells differentiate into adaxial (C and F; arrowhead) or lateral floor plate (D and G; arrowhead) cells in the pancreatic region, i.e. between the 1st and 4th somites.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 14(4), Chung, W.S., and Stainier, D.Y., Intra-endodermal interactions are required for pancreatic beta cell induction, 582-593, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell