Fig. 3

Fig. 3 Injection of truncated bmpr2a and bmpr2b mRNA induce BMP loss-of-function phenotypes. Morphological phenotypes were classified according to Kishimoto and colleagues (Kishimoto et al., 1997). (A) Uninjected embryo at tailbud stage. Embryos were injected with 100 ng/μl of bmpr2a-Δic (B, E, H) or 100 ng/μl of bmpr2b-Δic (C, F, I). Injection of bmpr2a-Δic (B) or bmpr2b-Δic (C) induced an enlarged notochord at tailbud stage (red arrows). (D) Wild type embryo at 24 hpf. (E) Example of C2–C4 dorsalized phenotypes after injection of bmpr2a-Δic mRNA and (F) C2–C3 phenotypes after injection of bmpr2b-Δic mRNA. (G) Wild type tail at 24 hpf. (H) Partial tail duplication in bmpr2a-Δic-injected embryo. (I) Cloacal cysts appear frequently upon bmpr2a-Δic or bmpr2b-Δic injection. Anterior is to the left (A–I). Quantification of the number of embryos showing dorsalized phenotypes in each category (C1–C5) upon injection of 50 and 100 ng/μl bmpr2a-Δic (J) or bmpr2b-Δic mRNA (K). n - number of injected embryos at each concentration.