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Fig. 2 In situ expression analysis of transcripts possibly involved during cartilage differentiation. A-J: Expression of five cartilage genes at 3 days postamputation (dpa; A-E) and 5 dpa (F-J). Arrowheads represent the site of amputation. The rug1, prohibitin, ENTPD5. and osterix are expressed in a region adjacent to the blastema (asterisks). The prohibitin and ENTPD5 show additional expression in a more proximal region of the regenerates. The expression of biglycan is seen only in the proximal region. A slight staining of biglycan in the blastema seems to be a background signal. K-R: Expression of cartilage genes during the embryonic and larval development. K-N: At stage 39, the expression of the four genes, except biglycan, is observed in the developing cartilage of branchial arches (arrowheads). O-R: At stage 39, the expression in the fin primordia begins in the basal region of median fin fold (arrows), at which location the cartilage of hemirays (lepidotrichium) is differentiating from the osteoblasts.