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Fig. 2 Effect of simulated microgravity on hsp70 mRNA expression. After the exposure to simulated microgravity, 3 µg of total RNA samples from the embryos of each control group (C) and experimental group (mg) were loaded on each lane and Northern blot analysis for hsp70 mRNA was performed (upper panel). To confirm the integrity of RNA and the transfer efficiency, ribosomal RNAs on a nylon membrane stained with methylene blue are shown in the lower panel. The numbers on the bottom indicate the normalized densitometry of each group. This experiment was performed in triplicate.
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