Effectiveness of amotl2 morphants. Embryos injected with 50 pg amotl2-GFP plasmid DNA showed green fluorescence at the shield stage (A). When co-injected with amotl2MO1 (MO1) or amotl2MO2 (MO2), green fluorescence decreased in a dose-dependent way (B-D,F-H). However, co-injection with cMO1 did not inhibit production of Amotl2-GFP fusion protein (E). Note that a higher dose of MO2 was required to inhibit Amotl2-GFP expression.
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