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Fig. 4 Characterization of E-box mutations on the activity of smyd1 promoter by transient expression analysis in zebrafish embryos. All panels are side views showing green fluorescent protein (GFP) expression in skeletal muscles of zebrafish embryos at 38 hours postfertilization (hpf). From A to H, zebrafish embryos were injected with the following DNA constructs: A: [smyd1-gfp (0.5)]; B: [smyd1-gfp (a*)]; C: [smyd1-gfp (a*b*)]; D: [smyd1-gfp (a*b*c*)]; E: [smyd1-gfp (a*b*c*d*)]; F: [smyd1-gfp (a*b*c*d*e*)], G: [smyd1-gfp (a*b*c*d*e*f*)], and H: [smyd1-gfp (a*b*c*d*e*f*g*)], respectively. Scale bar = 80 μm.