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Fig. 5 flh mutant cells can make notochord when transplanted into a wild-type environment. Blastula transplantations were performed as described in the Materials and Methods. (A-D) Lateral views of transplants at 24-28 h; shh-expressing cells are shown in blue and the transplanted cells are shown in brown. (A) A control transplant showing transplanted wild-type cells contributing to wild-type host notochord (concave arrowheads) and floor plate (arrowheads). (B) A transplant showing flh- cells contributing to wild-type host notochord and floor plate. (C) A transplant showing contribution of flh- cells to wild-type host floorplate, but not to the notochord. (D) The same embryo as in C, but a different focal plane to show the large number of flh- donor muscle cells (asterisks). (E-H) Images of wild-type (E,F) and flh- (G,H) transplanted cells (shown in red) in live wildtype hosts. Embryos were photographed several times during the segmentation period, beginning at the 3-somite stage (11 h) (E,G) and ending at the 20-somite stage (19 h) (F,H). Scale bars, 50 um (AD), 25 um (E-H).