ZFIN Gene: nsfb

ZFIN ID: ZDB-GENE-050808-1

ZFIN ID: ZDB-GENE-050808-1

Gene Name:

N-ethylmaleimide-sensitive factor b

Gene Symbol:

nsfb Nomenclature History

Previous Names:

unm_au13, unm_au18

Gene Type:

protein_coding_gene

Location:

Chr: 12 Mapping Details/Browsers

Description:

Predicted to have ATPase activity. Involved in pigment granule maturation and retinal pigment epithelium development. Predicted to localize to Golgi stack. Is expressed in several structures, including nervous system; otic vesicle; pectoral fin; pectoral fin bud; and swim bladder. Used to study oculocutaneous albinism. Orthologous to human NSF (N-ethylmaleimide sensitive factor, vesicle fusing ATPase).

Genome Resources:

Alliance (1), Gene:554200 (1), Ensembl(GRCz11):ENSDARG00000038991 (1)

GENE EXPRESSION
All Expression Data:	4 figures from 3 publications
Wild-type Stages, Structures:	Pharyngula:Prim-5 (24.0h-30.0h) to Adult (90d-730d, breeding adult)
	brain eye gill head inner ear muscle nervous system neuromast otic vesicle pectoral fin pectoral fin bud somite swim bladder whole organism

MUTATIONS AND SEQUENCE TARGETING REAGENTS

Allele	Type	Localization	Consequence	Mutagen	Suppliers
au13	Unknown	Unknown	Unknown	ENU
au18	Point Mutation	Unknown	Premature Stop	ENU
hi2869Tg	Transgenic Insertion	Intron 18	Unknown	DNA	Zebrafish International Resource Center (ZIRC) (order this)
sa10323	Point Mutation	Unknown	Premature Stop	ENU	European Zebrafish Resource Center (EZRC) (order this) Zebrafish International Resource Center (ZIRC) (order this)
sa22087	Point Mutation	Unknown	Premature Stop	ENU	European Zebrafish Resource Center (EZRC) (order this) Zebrafish International Resource Center (ZIRC) (order this)
sa42022	Point Mutation	Unknown	Splice Site	ENU	Zebrafish International Resource Center (ZIRC) (order this)
sa42023	Point Mutation	Unknown	Splice Site	ENU	European Zebrafish Resource Center (EZRC) (order this) Zebrafish International Resource Center (ZIRC) (order this)

Sequence Targeting Reagents

Targeting Reagent	Created Alleles	Publications
MO1-nsfb	N/A	1

PHENOTYPE

Data:	7 figures from 4 publications
Observed in:	brain eye melanocyte eye lens melanocyte melanosome neuromast pigment cell retinal pigmented epithelium melanosome retinal pigmented epithelium whole organism

DISEASE ASSOCIATED WITH nsfb HUMAN ORTHOLOG No data available

DISEASE ASSOCIATED WITH nsfb VIA EXPERIMENTAL MODELS

Human Disease	Fish	Conditions	Citations
oculocutaneous albinism	nsfb^au13/au13	standard conditions	Hanovice et al., 2015
	nsfb^au18/au18(AB)	standard conditions	Hanovice et al., 2015

GENE ONTOLOGY

Ontology	GO Term
Biological Process	pigment granule maturation (more)
Cellular Component	Golgi stack (more)
Molecular Function	ATPase activity (more)

GO Terms (all 10)

PROTEIN FAMILIES, DOMAINS AND SITES No links to external sites available

TRANSCRIPTS
Type	Name	Length (nt)	Analysis
mRNA	nsfb-201 (1)	2353	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
	nsfb-203 (1)	3466	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
ncRNA	nsfb-003 (1)	281	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT

Browsers: UCSC, NCBI, Ensembl, ZFIN

INTERACTIONS AND PATHWAYS

ANTIBODIES No data available

PLASMIDS No data available

CONSTRUCTS WITH SEQUENCES FROM nsfb No data available

MARKER RELATIONSHIPS

nsfb Contained in:	[BAC] CH211-177M15 (1), DKEY-38P12 (1) (order this)
nsfb Encodes:	[cDNA] MGC:194633 (1), MGC:194652 (1)

SEQUENCE INFORMATION

Type	Accession #	Length (nt/aa)	Analysis
RNA	RefSeq:NM_001024454 (1)	2400 nt	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Genomic	GenBank:AL929346 (1)	232537 nt	Blast at MegaBLAST
Polypeptide	UniProtKB:B3DHK0 (1)	747 aa	Select Tool ZFIN BLAST SIB BLAST Ensembl Protein Blast

Sequence Information (all 27)

ORTHOLOGY for nsfb ( Chr: 12 )

CITATIONS (32)

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