EST

IMAGE:6893873

ID
ZDB-EST-060810-74
Name
IMAGE:6893873
Symbol
IMAGE:6893873
Previous Names
  • FR_3603 (1)
Type
EST
Location
Unmapped
Genome Resources
None
Species
Danio rerio
Library
GISZF001_ra
Digest
Insert Size
2000
Cloning Site
Sfi A - Sfi B
Vector Type
plasmid
Vector
pDNR-LIB
Polymerase
T3 RNA polymerase
PCR Amplification
Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGG ATA ACC GTA TTA CCG CC 3'
5' CGC GCA ATT AAC CCT CAC TAA AGC ACT AGT CAT ACC AGG ATC 3'
Quality
( Probe is difficult to use. Generally basal level of expression with more intense labeling in particular structure. )
Protocol
Thisse in situ hybridization protocol
Note
None
Gene Expression
Directly Submitted Expression Data
1 figure (1 image) from Thisse et al., 2004 [IMAGE:6893873]
Marker Relationships
Sequences
Citations