EST Name: IMAGE:5411761
Sequence Ontology ID : SO:0000345
Previous Name: FR_6814B (1)

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Species: Danio rerio
Library: Sugano SJD adult male
Digest:
Insert Size: 3000
Cloning Site: EcoRI-PstI
Vector Type: Plasmid
Vector: pME18S-FL3
Polymerase: T3 RNA polymerase
PCR Amplification: Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGTACGGAAGTGTTACTTCTGCTC3'
5'GGATCCATTAACCCTCACTAAAGGGAAGGCCGCGACCTGCAGCTC3'
Quality: ( Probe is difficult to use. Generally basal level of expression with more intense labeling in particular structure. )
Protocol: Thisse in situ hybridization protocol
Location: Unmapped
GBROWSE
GENE EXPRESSION
Directly Submitted Expression Data: 1 figure (1 image) from Thisse et al., 2004 [IMAGE:5411761]
MARKER RELATIONSHIPS
IMAGE:5411761 Is Encoded by: [Gene] pus1 (1)
SEQUENCE INFORMATION
Type Accession # Length (nt/aa) Analysis
RNA GenBank:BM104700 (1) 631 nt
Genome Resources:
CITATIONS (2)