IMAGE:7145382

ID

ZDB-EST-050506-39

Name

IMAGE:7145382

Symbol

IMAGE:7145382

Previous Names

• FR_2351 (1)

Type

EST

Location

Unmapped

Genome Resources

None

Species

Danio rerio

Library

GISZF001_ra

Digest

Insert Size

1900

Cloning Site

Sfi A - Sfi B

Vector Type

plasmid

Vector

pDNR-LIB

Polymerase

T3 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGG ATA ACC GTA TTA CCG CC 3'
5' CGC GCA ATT AAC CCT CAC TAA AGC ACT AGT CAT ACC AGG ATC 3'

Suppliers

None

Protocol

Thisse in situ hybridization protocol

Note

It is not possible to determine which of the two genes this EST matches. The genes map to different positions on the same chromosome and have almost identical coding regions. As a probe this sequence would probably hybridize to both genes giving a composite expression pattern.