| EST Name: |
IMAGE:7146157
|
| Sequence Ontology ID : |
SO:0000345 |
|
Previous Name:
|
FR2466 (1)
|
Attributions for Alias: {{control.newAlias}}
Delete Alias:
(Including Attributions)
|
| Species: |
Danio rerio |
| Library: |
GISZF001_ra |
| Digest: |
|
| Insert Size: |
2000 |
| Cloning Site: |
Sfi A - Sfi B |
| Vector Type: |
plasmid |
| Vector: |
pDNR-LIB |
| Polymerase: |
T3 RNA polymerase |
| PCR Amplification: |
Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGG ATA ACC GTA TTA CCG CC 3'
5' CGC GCA ATT AAC CCT CAC TAA AGC ACT AGT CAT ACC AGG ATC 3' |
| Source: |
|
| Protocol: |
Thisse in situ hybridization protocol
|
| Location: |
Unmapped
|
