CRISPR

CRISPR3-ripk3

ID

ZDB-CRISPR-210819-5

Name

CRISPR3-ripk3

Previous Names

None

Target

ripk3 (1)

Sequence

5' - GGAGGGCGAGAGCACACGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
szy12	ripk3
vu701	ripk3
vu702	ripk3

Expression

Gene expression in Wild Types + CRISPR3-ripk3

No data available

Phenotype

Phenotype resulting from CRISPR3-ripk3

No data available

Phenotype of all Fish created by or utilizing CRISPR3-ripk3

Phenotype	Fish	Conditions	Figures
whole organism il1b expression increased amount, abnormal	ripk3^szy12/szy12	chemical treatment by injection: lipopolysaccharide	Fig. 5 from Wen et al., 2022
whole organism il10 expression amount, ameliorated	ripk3^szy12/szy12	transection: caudal fin	Fig. 4 from Wen et al., 2022
whole organism il4 expression increased amount, abnormal	ripk3^szy12/szy12	chemical treatment by injection: lipopolysaccharide	Fig. 5 from Wen et al., 2022
whole organism cxcl8a expression increased amount, abnormal	ripk3^szy12/szy12	transection: caudal fin	Fig. 4 from Wen et al., 2022
whole organism il1b expression amount, ameliorated	ripk3^szy12/szy12	transection: caudal fin	Fig. 4 from Wen et al., 2022
whole organism il13 expression increased amount, abnormal	ripk3^szy12/szy12	transection: caudal fin	Fig. 4 from Wen et al., 2022
whole organism il13 expression increased amount, abnormal	ripk3^szy12/szy12	chemical treatment by injection: lipopolysaccharide	Fig. 5 from Wen et al., 2022
whole organism il6 expression amount, ameliorated	ripk3^szy12/szy12	transection: caudal fin	Fig. 4 from Wen et al., 2022
whole organism il6 expression increased amount, abnormal	ripk3^szy12/szy12	chemical treatment by injection: lipopolysaccharide	Fig. 5 from Wen et al., 2022
whole organism il4 expression increased amount, abnormal	ripk3^szy12/szy12	transection: caudal fin	Fig. 4 from Wen et al., 2022
whole organism il10 expression increased amount, abnormal	ripk3^szy12/szy12	chemical treatment by injection: lipopolysaccharide	Fig. 5 from Wen et al., 2022
macrophage increased distance pancreatic B cell, abnormal	ripk3^vu701/vu701; gl22Tg; vu513Tg	nutrient increased	Fig. 5 from Yang et al., 2020
whole organism glucose normal amount, ameliorated	ripk3^vu701/vu701; vu513Tg	nutrient increased	Fig. 2 from Yang et al., 2020
pancreatic B cell normal amount, ameliorated	ripk3^vu701/vu701; vu513Tg	nutrient increased	Fig. 2 from Yang et al., 2020
macrophage increased distance pancreatic B cell, abnormal	ripk3^vu702/vu702; gl22Tg; vu513Tg	nutrient increased	Fig. 5 from Yang et al., 2020
pancreatic B cell normal amount, ameliorated	ripk3^vu702/vu702; vu513Tg	nutrient increased	Fig. 2 from Yang et al., 2020
whole organism glucose normal amount, ameliorated	ripk3^vu702/vu702; vu513Tg	nutrient increased	Fig. 2 from Yang et al., 2020

Citations