CRISPR

CRISPR2-atg7

ID

ZDB-CRISPR-200806-1

Name

CRISPR2-atg7

Previous Names

None

Target

Sequence

5' - GCTTCACAGCTGGATGTTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hza54	atg7
hza55	atg7

Expression

Gene expression in Wild Types + CRISPR2-atg7

No data available

Phenotype

Phenotype resulting from CRISPR2-atg7

No data available

Phenotype of all Fish created by or utilizing CRISPR2-atg7

Phenotype	Fish	Conditions	Figures
whole organism dead, abnormal	atg7^hza54/hza54 (AB)	standard conditions	text only from Mawed et al., 2020
whole organism dead, abnormal	atg7^hza55/hza55 (AB)	standard conditions	text only from Mawed et al., 2020
male organism liver ab13-tp53 labeling increased amount, abnormal	atg7^hza54/+ (AB)	standard conditions	Figure 6 from Mawed et al., 2020
liver hepatic tumor increased tendency male organism, abnormal	atg7^hza54/+ (AB)	standard conditions	Figure 2 from Mawed et al., 2020
male organism decreased life span, abnormal	atg7^hza54/+ (AB)	standard conditions	Figure 2 from Mawed et al., 2020
male organism liver Ab6-atg5 labeling decreased amount, abnormal	atg7^hza54/+ (AB)	standard conditions	Figure 5 from Mawed et al., 2020
male organism liver ab13-tp53 labeling increased amount, abnormal	atg7^hza55/+ (AB)	standard conditions	Figure 6 from Mawed et al., 2020
male organism liver Ab6-atg5 labeling decreased amount, abnormal	atg7^hza55/+ (AB)	standard conditions	Figure 5 from Mawed et al., 2020
liver hepatic tumor increased tendency male organism, abnormal	atg7^hza55/+ (AB)	standard conditions	Figure 2 from Mawed et al., 2020
male organism decreased life span, abnormal	atg7^hza55/+ (AB)	standard conditions	Figure 2 from Mawed et al., 2020

Citations