CRISPR

CRISPR10-kmt2d

ID

ZDB-CRISPR-181017-3

Name

CRISPR10-kmt2d

Previous Names

None

Target

kmt2d (1)

Sequence

5' - GGGTGAGGTGCTGATAAACGTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was TGG at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
rdu1001	kmt2d

Expression

Gene expression in Wild Types + CRISPR10-kmt2d

No data available

Phenotype

Phenotype resulting from CRISPR10-kmt2d

Phenotype	Fish	Figures
convergent extension involved in gastrulation disrupted, abnormal	EKW + CRISPR10-kmt2d	Fig. S7 from Tsai et al., 2018
mandibular arch skeleton morphology, abnormal	EKW + CRISPR10-kmt2d	Fig. S7 from Tsai et al., 2018
Meckel's cartilage decreased distance ceratohyal cartilage, abnormal	EKW + CRISPR10-kmt2d	Fig. S7 from Tsai et al., 2018

Phenotype of all Fish created by or utilizing CRISPR10-kmt2d

Phenotype	Fish	Conditions	Figures
brain cell population proliferation process quality, ameliorated	kmt2d^{rdu1001/rdu1001}	chemical treatment by environment: B-Raf inhibitor	Fig. 6 from Tsai et al., 2018
convergent extension involved in gastrulation process quality, ameliorated	kmt2d^{rdu1001/rdu1001}	chemical treatment by environment: B-Raf inhibitor	Fig. 6 from Tsai et al., 2018
Meckel's cartilage decreased distance ceratohyal cartilage, abnormal	kmt2d^{rdu1001/rdu1001}	standard conditions	Fig. 6 from Tsai et al., 2018
mandibular arch skeleton morphology, abnormal	kmt2d^{rdu1001/rdu1001}	standard conditions	Fig. 6 from Tsai et al., 2018
mandibular arch skeleton morphology, ameliorated	kmt2d^{rdu1001/rdu1001}	chemical treatment by environment: B-Raf inhibitor	Fig. 6 from Tsai et al., 2018
brain cell population proliferation decreased occurrence, abnormal	kmt2d^{rdu1001/rdu1001}	standard conditions	Fig. 6 from Tsai et al., 2018
Meckel's cartilage distance ceratohyal cartilage, ameliorated	kmt2d^{rdu1001/rdu1001}	chemical treatment by environment: B-Raf inhibitor	Fig. 6 from Tsai et al., 2018
convergent extension involved in gastrulation disrupted, abnormal	kmt2d^{rdu1001/rdu1001}	standard conditions	Fig. 6 from Tsai et al., 2018
mandibular arch skeleton morphology, abnormal	EKW + CRISPR10-kmt2d	standard conditions	Fig. S7 from Tsai et al., 2018
convergent extension involved in gastrulation disrupted, abnormal	EKW + CRISPR10-kmt2d	standard conditions	Fig. S7 from Tsai et al., 2018
Meckel's cartilage decreased distance ceratohyal cartilage, abnormal	EKW + CRISPR10-kmt2d	standard conditions	Fig. S7 from Tsai et al., 2018
Meckel's cartilage distance ceratohyal cartilage, ameliorated	EKW + CRISPR10-kmt2d	chemical treatment by environment: B-Raf inhibitor	Fig. S7 from Tsai et al., 2018
convergent extension involved in gastrulation process quality, ameliorated	EKW + CRISPR10-kmt2d	chemical treatment by environment: B-Raf inhibitor	Fig. S7 from Tsai et al., 2018
mandibular arch skeleton morphology, ameliorated	EKW + CRISPR10-kmt2d	chemical treatment by environment: B-Raf inhibitor	Fig. S7 from Tsai et al., 2018

Citations