CRISPR

CRISPR5-dhx15

ID

ZDB-CRISPR-160128-282

Name

CRISPR5-dhx15

Previous Names

Z000367 (1)

Target

dhx15 (1)

Sequence

5' - GGCTGCAGTTGCCCGTCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

CRISPRz:Z000367 (1)

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hg92	dhx15

Expression

Gene expression in Wild Types + CRISPR5-dhx15

No data available

Phenotype

Phenotype resulting from CRISPR5-dhx15

No data available

Phenotype of all Fish created by or utilizing CRISPR5-dhx15

Phenotype	Fish	Conditions	Figures
whole organism dead, abnormal	dhx15^hg92/hg92	standard conditions	Fig. S3 from McElderry et al., 2019
post-vent region increased curvature, abnormal	dhx15^hg92/hg92	standard conditions	Fig. 3 from McElderry et al., 2019
whole organism ab11-tp53 labeling increased amount, abnormal	dhx15^hg92/hg92	standard conditions	Fig. 5 from McElderry et al., 2019
mRNA splicing, via spliceosome process quality, abnormal	dhx15^hg92/hg92	standard conditions	Fig. 5 from McElderry et al., 2019
head apoptotic process increased occurrence, abnormal	dhx15^hg92/hg92	standard conditions	Fig. 6 from McElderry et al., 2019
pericardium edematous, abnormal	dhx15^hg92/hg92	standard conditions	Fig. 3 from McElderry et al., 2019
alternative mRNA splicing, via spliceosome increased process quality, abnormal	dhx15^hg92/hg92	standard conditions	Fig. 5 from McElderry et al., 2019
head decreased size, abnormal	dhx15^hg92/hg92	standard conditions	Fig. 3 from McElderry et al., 2019
head apoptotic, abnormal	dhx15^hg92/hg92	standard conditions	Fig. 6 from McElderry et al., 2019
pectoral fin deformed, abnormal	dhx15^hg92/hg92	standard conditions	Fig. 3 from McElderry et al., 2019
whole organism mdm2 expression increased amount, abnormal	dhx15^hg92/hg92	standard conditions	Fig. 5 from McElderry et al., 2019
whole organism tp53 expression increased amount, abnormal	dhx15^hg92/hg92	standard conditions	Fig. 5 from McElderry et al., 2019
head apoptotic process increased occurrence, abnormal	dhx15^hg92/hg92; tp53^hg91/hg91	standard conditions	Fig. 6 from McElderry et al., 2019
head apoptotic, abnormal	dhx15^hg92/hg92; tp53^hg91/hg91	standard conditions	Fig. 6 from McElderry et al., 2019

Citations