MGC:66347

ID

ZDB-CDNA-040425-3634

Name

MGC:66347

Symbol

MGC:66347

Previous Names

• FR616 (1)

Type

cDNA

Location

Unmapped

Genome Resources

None

Clone Problem Type

CHIMERIC

Species

Danio rerio

Library

Sugano SJD adult male

Digest

Insert Size

1976

Cloning Site

DraIII

Vector Type

Plasmid

Vector

pME18S-FL3

Polymerase

T3 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGT ACG GAA GTG TTA CTT CTG CTC 3'
5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GGC CGC GAC CTG CAG CTC 3'

Suppliers

None

Protocol

Thisse in situ hybridization protocol

Note

None