| cDNA Name: |
MGC:55191
|
| Sequence Ontology ID : |
SO:0000756 |
|
Previous Name:
|
FR5013 (1)
|
Attributions for Alias: {{control.newAlias}}
Delete Alias:
(Including Attributions)
|
| Species: |
Danio rerio |
| Library: |
Sugano SJD adult male |
| Digest: |
|
| Insert Size: |
2863 |
| Cloning Site: |
EcoRI-PstI |
| Vector Type: |
Plasmid |
| Vector: |
pME18S-FL3 |
| Polymerase: |
T3 RNA polymerase |
| PCR Amplification: |
Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGTACGGAAGTGTTACTTCTGCTC3'
5'GGATCCATTAACCCTCACTAAAGGGAAGGCCGCGACCTGCAGCTC3' |
| Source: |
|
| Protocol: |
Thisse in situ hybridization protocol
|
| Location: |
Unmapped
|
