MGC:56505

ID

ZDB-CDNA-040425-2794

Name

MGC:56505

Symbol

MGC:56505

Previous Names

• FR4954 (1)

Type

cDNA

Location

Unmapped

Genome Resources

None

Clone Problem Type

CHIMERIC

Species

Danio rerio

Library

Sugano SJD adult male

Digest

Insert Size

1582

Cloning Site

EcoRI-PstI

Vector Type

Plasmid

Vector

pME18S-FL3

Polymerase

T3 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TGTACGGAAGTGTTACTTCTGCTC3'
5'GGATCCATTAACCCTCACTAAAGGGAAGGCCGCGACCTGCAGCTC3'

Suppliers

None

Protocol

Thisse in situ hybridization protocol

Note

Analysis of the sequences associated with the clone suggest that it may be a chimeric clone represented by sequences derived from the zgc:77704 gene and from another genomic region. mRNA similar to glutaryl-Coenzyme A dehydrogenase (cDNA clone MGC:56505 )