ZFIN Lab: Winkler Lab

ZFIN ID: ZDB-LAB-020529-1

Winkler Lab

PI/Director:	Winkler, Christoph
Contact Person:	Winkler, Christoph
Email:	dbswcw@nus.edu.sg
URL:	http://www.dbs.nus.edu.sg/lab/winkler_lab/home.html
Address:	Department of Biological Sciences National University of Singapore 14 Science Drive 4, S2-04 Singapore 117543
Country:	Singapore
Phone:	0065-6516 7376
Fax:	0065-6779 2486
Line Designation:	ns

GENOMIC FEATURES ORIGINATING FROM THIS LAB

Show all 15 genomic features

STATEMENT OF RESEARCH INTERESTS

We use zebrafish and medaka models to investigate fundamental processes of embryonic development and to approach the molecular basis of different human disorders. This includes:
• Formation and patterning of the central nervous system
• Molecular mechanisms of neural degeneration
• Development of somites and bones
• Fish models for human neurodegenerative disorders and bone diseases

LAB MEMBERS

Buettner, Anita Post-Doc	Phan, Quang Tien Post-Doc	Thanh, Thuy Post-Doc
Graf, Martin Graduate Student	Kelvin, See Zhenwei Graduate Student	Rajaei, Flora Graduate Student
Yao, Sheng Graduate Student	Meng, Yew Hong	Pearl, Cheong Simin

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Tan, W.H., Winkler, C. (2024) Lineage Tracing of Bone Cells in the Regenerating Fin and During Repair of Bone Lesions. Methods in molecular biology (Clifton, N.J.). 2707:9911099-110

Tzung, K.W., Lalonde, R.L., Prummel, K.D., Mahabaleshwar, H., Moran, H.R., Stundl, J., Cass, A.N., Le, Y., Lea, R., Dorey, K., Tomecka, M.J., Zhang, C., Brombacher, E.C., White, W.T., Roehl, H.H., Tulenko, F.J., Winkler, C., Currie, P.D., Amaya, E., Davis, M.C., Bronner, M.E., Mosimann, C., Carney, T.J. (2023) A median fin derived from the lateral plate mesoderm and the origin of paired fins. Nature. 618(7965):543-549

Ethiraj, L.P., Fong, E.L.S., Liu, R., Chan, M., Winkler, C., Carney, T.J. (2022) Colorimetric and fluorescent TRAP assays for visualising and quantifying fish osteoclast activity. European journal of histochemistry : EJH. 66(2)

Koh, A., Sarusie, M.V., Ohmer, J., Fischer, U., Winkler, C., Wohland, T. (2021) Fluorescence Correlation Spectroscopy Reveals Survival Motor Neuron Oligomerization but No Active Transport in Motor Axons of a Zebrafish Model for Spinal Muscular Atrophy. Frontiers in cell and developmental biology. 9:639904

Tay, S.H., Ellieyana, E.N., Le, Y., Sarusie, M.V., Grimm, C., Ohmer, J., Mathuru, A., Fischer, U., Winkler, C. (2021) A novel zebrafish model for intermediate type spinal muscular atrophy demonstrates importance of Smn for maintenance of mature motor neurons. Human molecular genetics. 30(24):2488-2502

Foo, Y.Y., Motakis, E., Tiang, Z., Shen, S., Lai, J.K.H., Chan, W.X., Wiputra, H., Chen, N., Chen, C.K., Winkler, C., Foo, R.S.Y., Yap, C.H. (2021) Effects of Extended Pharmacological Disruption of Zebrafish Embryonic Heart Biomechanical Environment on Cardiac Function, Morphology and Gene Expression. Developmental Dynamics : an official publication of the American Association of Anatomists. 250(12):1759-1777

Fraher, D., Mann, R.J., Dubuisson, M.J., Ellis, M.K., Yu, T., Walder, K., Ward, A.C., Winkler, C., Gibert, Y. (2021) The endocannabinoid system and retinoic acid signaling combine to influence bone growth. Molecular and Cellular Endocrinology. 529:111267

Koh, A., Tao, S., Jing, G.Y., Chaganty, V., See, K., Purushothaman, K., Orbán, L., Mathuru, A.S., Wohland, T., Winkler, C. (2020) A Neurexin2aa deficiency results in axon pathfinding defects and increased anxiety in zebrafish. Human molecular genetics. 29(23):3765-3780

Foo, Y.Y., Pant, S., Tay, H.S., Imangali, N., Chen, N., Winkler, C., Yap, C.H. (2019) 4D modelling of fluid mechanics in the zebrafish embryonic heart. Biomechanics and Modeling in Mechanobiology. 19(1):221-232

Dasyani, M., Tan, W.H., Sundaram, S., Imangali, N., Centanin, L., Wittbrodt, J., Winkler, C. (2019) Lineage tracing of col10a1 cells identifies distinct progenitor populations for osteoblasts and joint cells in the regenerating fin of medaka (Oryzias latipes). Developmental Biology. 455(1):85-99

Lleras-Forero, L., Winkler, C., Schulte-Merker, S. (2019) Zebrafish and medaka as models for biomedical research of bone diseases. Developmental Biology. 457(2):191-205

Phan, Q.T., Sipka, T., Gonzalez, C., Levraud, J.P., Lutfalla, G., Nguyen-Chi, M. (2018) Neutrophils use superoxide to control bacterial infection at a distance. PLoS pathogens. 14:e1007157

Sheng, D., See, K., Hu, X., Yu, D., Wang, Y., Liu, Q., Li, F., Lu, M., Zhao, J., Liu, J. (2018) Disruption of LRRK2 in Zebrafish leads to hyperactivity and weakened antibacterial response. Biochemical and Biophysical Research Communications. 497(4):1104-1109

Tan, W.H., Witten, P.E., Winkler, C., Au, D.W.T., Huysseune, A. (2017) Telomerase Expression in Medaka ( Oryzias melastigma) Pharyngeal Teeth. Journal of dental research. 96:678-684

Yu, T., Graf, M., Renn, J., Schartl, M., Larionova, D., Huysseune, A., Witten, P.E., Winkler, C. (2017) A vertebrate-specific and essential role for osterix in osteogenesis revealed by gene knockout in the teleost medaka. Development (Cambridge, England). 144:265-271

Witten, P.E., Harris, M.P., Huysseune, A., Winkler, C. (2017) Small teleost fish provide new insights into human skeletal diseases. Methods in cell biology. 138:321-346

Yu, T., Winkler, C. (2017) Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model. Journal of visualized experiments : JoVE. (119)

Spiró, Z., Koh, A., Tay, S., See, K., Winkler, C. (2016) Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Scientific Reports. 6:27470

Nguyen Chi, M., Laplace-Builhe, B., Travnickova, J., Luz-Crawford, P., Tejedor, G., Phan, Q.T., Duroux-Richard, I., Levraud, J.P., Kissa, K., Lutfalla, G., Jorgensen, C., Djouad, F. (2015) Identification of polarized macrophage subsets in zebrafish. eLIFE. 4:e07288

Willems, B., Tao, S., Yu, T., Huysseune, A., Witten, P.E., Winkler, C. (2015) The Wnt Co-Receptor Lrp5 Is Required for Cranial Neural Crest Cell Migration in Zebrafish. PLoS One. 10:e0131768

Quach, H.N., Tao, S., Vrljicak, P., Joshi, A., Ruan, H., Sukumaran, R., Varshney, G.K., LaFave, M.C., Screen Team, T.D., Burgess, S.M., Winkler, C., Emelyanov, A., Parinov, S., Sampath, K. (2015) A Multifunctional Mutagenesis System for Analysis of Gene Function in Zebrafish. G3 (Bethesda). 5(6):1283-99

Graf, M., Teo Qi-Wen, E.R., Viktor Sarusie, M., Rajaei, F., Winkler, C. (2015) Dmrt5 controls corticotrope and gonadotrope differentiation in the zebrafish pituitary. Molecular endocrinology (Baltimore, Md.). 29(2):187-99

Linder, B., Hirmer, A., Gal, A., Rüther, K., Bolz, H.J., Winkler, C., Laggerbauer, B., Fischer, U. (2014) Identification of a PRPF4 Loss-of-Function Variant That Abrogates U4/U6.U5 Tri-snRNP Integration and Is Associated with Retinitis Pigmentosa. PLoS One. 9:e111754

Nguyen-Chi, M., Phan, Q.T., Gonzalez, C., Dubremetz, J.F., Levraud, J.P., Lutfalla, G. (2014) Transient infection of the zebrafish notochord with E. coli induces chronic inflammation. Disease models & mechanisms. 7:871-882

See, K., Yadav, P., Giegerich, M., Cheong, P.S., Graf, M., Vyas, H., Lee, S.G., Mathavan, S., Fischer, U., Sendtner, M., and Winkler, C. (2014) SMN deficiency alters Nrxn2 expression and splicing in zebrafish and mouse models of spinal muscular atrophy. Human molecular genetics. 23(7):1754-70

Winkler, C., and Yao, S. (2014) The midkine family of growth factors: Diverse roles in nervous system formation and maintenance. British journal of pharmacology. 171(4):905-12

Haendeler, J., Mlynek, A., Büchner, N., Lukosz, M., Graf, M., Güttler, C., Jakob, S., Farrokh, S., Kunze, K., Goy, C., Guardiola-Serrano, F., Schaal, H., Cortese-Krott, M., Deenen, R., Köhrer, K., Winkler, C., and Altschmied, J. (2013) Two Isoforms of Sister-of-Mammalian Grainyhead Have Opposing Functions in Endothelial Cells and In Vivo. Arterioscler. Thromb. Vasc. Biol.. 33(7):1639-1646

Yao, S., Cheng, M., Zhang, Q., Wasik, M., Kelsh, R., and Winkler, C. (2013) Anaplastic lymphoma kinase is required for neurogenesis in the developing central nervous system of zebrafish. PLoS One. 8(5):e63757

Lim, J., Yao, S., Graf, M., Winkler, C., and Yang, D. (2013) Structure-function analysis of full-length midkine reveals novel residues important for heparin-binding and zebrafish embryogenesis. The Biochemical journal. 451(3):407-15

Amali, A.A., Sie, L., Winkler, C., and Featherstone, M. (2013) Zebrafish hoxd4a Acts Upstream of meis1.1 to Direct Vasculogenesis, Angiogenesis and Hematopoiesis. PLoS One. 8(3):e58857

Tran, L.D., Hino, H., Quach, H., Lim, S., Shindo, A., Mimori-Kiyosue, Y., Mione, M., Ueno, N., Winkler, C., Hibi, M., and Sampath, K. (2012) Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish. Development (Cambridge, England). 139(19):3644-3652

Yin, J., Brocher, J., Linder, B., Hirmer, A., Sundaramurthi, H., Fischer, U., and Winkler, C. (2012) The 1D4 antibody labels outer segments of long double cone but not rod photoreceptors in zebrafish. Investigative ophthalmology & visual science. 53(8):4943-4951

Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T. (2012) The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development. Developmental Biology. 363(2):438-450

Yin, J., Brocher, J., Fischer, U., and Winkler, C. (2011) Mutant Prpf31 causes pre-mRNA splicing defects and rod photoreceptor cell degeneration in a zebrafish model for Retinitis pigmentosa. Molecular neurodegeneration. 6(1):56

Li, Z.H., Alex, D., Siu, S.O., Chu, I.K., Renn, J., Winkler, C., Lou, S., Tsui, S.K., Zhao, H.Y., Yan, W.R., Mahady, G.B., Li, G.H., Kwan, Y.W., Wang, Y.T., and Lee, S.M. (2011) Combined in vivo imaging and omics approaches reveal metabolism of icaritin and its glycosides in zebrafish larvae. Molecular Biosystems. 7(7):2128-38

Linder, B., Dill, H., Hirmer, A., Brocher, J., Lee, G.P., Mathavan, S., Bolz, H.J., Winkler, C., Laggerbauer, B., and Fischer, U. (2011) Systemic splicing factor deficiency causes tissue-specific defects: A zebrafish model for Retinitis pigmentosa. Human molecular genetics. 20(2):368-377

Cheah, F.S., Winkler, C., Jabs, E.W., and Chong, S.S. (2010) tgfbeta3 Regulation of Chondrogenesis and Osteogenesis in Zebrafish is Mediated Through Formation and Survival of a Subpopulation of the Cranial Neural Crest. Mechanisms of Development. 128(7-8):329-344

Glinka, M., Herrmann, T., Funk, N., Havlicek, S., Rossoll, W., Winkler, C., and Sendtner, M. (2010) The Heterogeneous Nuclear Ribonucleoprotein R is Necessary for Axonal {beta}-actin mRNA Translocation in Spinal Motor neurons. Human molecular genetics. 19(10):1951-1966

Hong, N., Li, M., Zeng, Z., Yi, M., Deng, J., Gui, J., Winkler, C., Schartl, M., and Hong, Y. (2010) Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos. Cellular and molecular life sciences : CMLS. 67(7):1189-1202

Yeo, G.H., Cheah, F.S., Winkler, C., Jabs, E.W., Venkatesh, B., and Chong, S.S. (2009) Phylogenetic and evolutionary relationships and developmental expression patterns of the zebrafish twist gene family. Development genes and evolution. 219(6):289-300

Spoorendonk, K.M., Peterson-Maduro, J., Renn, J., Trowe, T., Kranenbarg, S., Winkler, C., and Schulte-Merker, S. (2008) Retinoic acid and Cyp26b1 are critical regulators of osteogenesis in the axial skeleton. Development (Cambridge, England). 135(22):3765-3774

Liedtke, D., and Winkler, C. (2008) Midkine-b regulates cell specification at the neural plate border in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(1):62-74

To, T.T., Hahner, S., Nica, G., Rohr, K.B., Hammerschmidt, M., Winkler, C., and Allolio, B. (2007) Pituitary-interrenal interaction in zebrafish interrenal organ development. Molecular endocrinology (Baltimore, Md.). 21(2):472-485

Schafer, M., Kinzel, D., and Winkler, C. (2007) Discontinuous organization and specification of the lateral floor plate in zebrafish. Developmental Biology. 301(1):117-129

Renn, J., Winkler, C., Schartl, M., Fischer, R., and Goerlich, R. (2006) Zebrafish and medaka as models for bone research including implications regarding space-related issues. Protoplasma. 229(2-4):209-214

Schild-Prufert, K., Giegerich, M., Schafer, M., Winkler, C., and Krohne, G. (2006) Structural and functional characterization of the zebrafish lamin B receptor. European journal of cell biology. 85(8):813-824

Gajewski, M., Elmasri, H., Girschick, M., Sieger, D., and Winkler, C. (2006) Comparative analysis of her genes during fish somitogenesis suggests a mouse/chick-like mode of oscillation in medaka. Development genes and evolution. 216(6):315-332

Renn, J., Schaedel, M., Volff, J.N., Goerlich, R., Schartl, M., and Winkler, C. (2006) Dynamic expression of sparc precedes formation of skeletal elements in the Medaka (Oryzias latipes). Gene. 372:208-218

Sieger, D., Ackermann, B., Winkler, C., Tautz, D., and Gajewski, M. (2006) her1 and her13.2 are jointly required for somitic border specification along the entire axis of the fish embryo. Developmental Biology. 293(1):242-251

Bollig, F., Mehringer, R., Perner, B., Hartung, C., Schafer, M., Schartl, M., Volff, J.N., Winkler, C., and Englert, C. (2006) Identification and comparative expression analysis of a second wt1 gene in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(2):554-561

Winkler, C., Eggert, C., Gradl, D., Meister, G., Giegerich, M., Wedlich, D., Laggerbauer, B., and Fischer, U. (2005) Reduced U snRNP assembly causes motor axon degeneration in an animal model for spinal muscular atrophy. Genes & Development. 19(19):2320-2330

Schäfer, M., Rembold, M., Wittbrodt, J., Schartl, M., and Winkler, C. (2005) Medial floor plate formation in zebrafish consists of two phases and requires trunk-derived Midkine-a. Genes & Development. 19(8):897-902

Schäfer, M., Kinzel, D., Neuner, C., Schartl, M., Volff, J.N., and Winkler, C. (2005) Hedgehog and retinoid signalling confines nkx2.2b expression to the lateral floor plate of the zebrafish trunk. Mechanisms of Development. 122(1):43-56

Elmasri, H., Liedtke, D., Lücking, G., Volff, J.N., Gessler, M., and Winkler, C. (2004) her7 and hey1, but not lunatic fringe show dynamic expression during somitogenesis in medaka (Oryzias latipes). Gene expression patterns : GEP. 4(5):553-559

Prüfert, K., Winkler, C., Paulin-Levasseur, M., and Krohne, G. (2004) The lamina-associated polypeptide 2 (LAP2) genes of zebrafish and chicken: no LAP2alpha isoform is synthesised by non-mammalian vertebrates. European journal of cell biology. 83(8):403-411

Winkler, C., Elmasri, H., Klamt, B., Volff, J.N., and Gessler, M. (2003) Characterization of hey bHLH genes in teleost fish. Development genes and evolution. 213(11):541-553

Schoft, V.K., Beauvais, A.J., Lang, C., Gajewski, A., Prufert, K., Winkler, C., Akimenko, M.-A., Paulin-Levasseur, M., and Krohne, G. (2003) The lamina-associated polypeptide 2 (LAP2) isoforms {beta}, {gamma} and {omega} of zebrafish: developmental expression and behavior during the cell cycle. Journal of Cell Science. 116(12):2505-2517

Winkler, C., Schäfer, M., Duschl, J., Schartl, M., and Volff, J.-N. (2003) Functional divergence of two zebrafish midkine growth factors following fish-specific gene duplication. Genome research. 13:1067-1081

Hansen, I.A., To, T.T., Wortmann, S., Burmester, T., Winkler, C., Meyer, S.R., Neurner, C., Fassnacht, and Allolio, B. (2003) The pro-opiomelanocortin gene of the zebrafish (Danio rerio). Biochemical and Biophysical Research Communications. 303(4):1121-1128

Fischer, A., Leimeister, C., Winkler, C., Schumacher, N., Klamt, B., Elmasri, A., Steidl, C., Maier, M., Knobeloch, K.P., Amann, K., Helisch, A., Sendtner, M., and Gessler, M. (2002) Hey bHLH factors in cardiovascular development. Cold Spring Harbor symposia on quantitative biology. 67:63-70

Winkler, C. and Moon, R.T. (2001) Zebrafish mdk2, a novel secreted midkine, participates in posterior neurogenesis. Developmental Biology. 229(1):102-118

Götz, R., Köster, R.W., Winkler, C., Raulf, F., Lottspeich, F., Schartl, M., and Thoenen, H. (1994) Neurotrophin-6 is a new member of the nerve growth factor family. Nature. 372(6503):266-269