Graphical representation of the velocity (cm/s), distance to stimuli (cm), and turn angle (deg) results for both albino (A) and non-albino (N) zebrafish models in the predator video stimuli test. “1 h/24 h/48 h” represents the exposure time to oxytocin. The blue mark represents the moment when the stimulus appeared during the test. The data are expressed as the mean ± SEM, and a p < 0.05 was considered to be statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001).

Graphical representation of the freezing duration (seconds) and anxiety index (%) results for both albino (A) and non-albino (N) zebrafish models in the predator video stimuli test. “1 h/24 h/48 h” represents the exposure time to oxytocin. The blue mark represents the moment when the stimulus appeared during the test. The data are expressed as the mean ± SEM, and a p < 0.05 was considered to be statistically significant (* p < 0.05; ** p < 0.01).

Graphical representation of the results for both albino and non-albino zebrafish models in the conspecific video vs. real fish preference test ((A) Frecvency in screen zone non-albino; (B) Time spent in screen zone non-albino; (C) Distance to screen non-albino; (D) Frecvency in screen zone albino; (E) Time spent in screen zone albino; (F) Distance to screen albino). The data are expressed as the mean ± SEM, and a p < 0.05 was considered to be statistically significant (* p < 0.05).

Experimental setup of the predator video stimulus test. Image created in Biorender.comwww.biorender.com Accessed in 2 April 2024.

Experimental setup for the video conspecific vs. real fish preference test. Image created in Biorender.comwww.biorender.com Accessed in 2 April 2024.