FIGURE SUMMARY
Title

Study on the Function of Leptin Nutrient Acquisition and Energy Metabolism of Zebrafish (Danio rerio)

Authors
Wu, J., Zhuang, W., Lu, K., Zhang, L., Wang, Y., Chai, F., Liang, X.F.
Source
Full text @ Int. J. Mol. Sci.
Figure 1

(A,B) The sequence result of mutant genes. Dotted lines represent missing bases, and red squares represent inserted bases. The sgRNA sequences are highlighted in red, and the −5 bp and +8 bp deletions are indicated by sequencing validation.
Figure 2

Genee expression related to appetite and food intake of lepa−/−, lepb−/−, and wild-type zebrafish. (A) Food intake of lepa−/−, lepb−/−, and wild-type zebrafish. (B) Expression levels of appetite-related genes in the brain of the three genotypes of zebrafish. Different letters indicate significant differences (p < 0.05).
Figure 3

The effect of leptin on blood glucose and lipid levels. (A) Blood glucose levels, (B) plasma TG levels, and (C) plasma cholesterol levels in the three genotypes of zebrafish. Different letters indicate significant differences (p < 0.05), with n = 6 for each genotype.
Figure 4

Oil red O and AB-PAS staining of liver and muscle tissue of three genotypes zebrafish. (A) Oil red O staining of the liver. (B) Oil red O staining of muscle. (C) AB-PAS staining of the liver. (D) AB-PAS staining of muscle.
Figure 5

The levels of glycogen and triglyceride (TG) in the liver and muscle of three genotypes of zebrafish. (A) Glycogen levels in the liver. (B) Glycogen levels in muscle. (C) TG levels in the liver. (D) TG levels in muscle. The letters a, b, and c in the bar chart represent significant differences for each index among lepa−/−, lepb−/−, and wild-type zebrafish (p < 0.05), with n = 6 for each genotype.
Figure 6

Expression levels of genes related to glucose metabolism in the liver. (A) Glycolysis and gluconeogenesis genes. (B) Glycogen metabolism gene. The letters a, b, and c in the bar chart represent significant differences foreach index among lepa−/−, lepb−/−, and wild-type zebrafish (p < 0.05), with n = 6 for each genotype.
Figure 7

Expression levels of lipid metabolism genes in the liver (A) and muscle (B) of three genotypes of zebrafish. The letters a, b, and c in the bar chart represent significant differences for each index among lepa−/−, lepb−/−, and wild-type zebrafish (p < 0.05), with n = 6 for each genotype.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.
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